pAAV9(5)-hSyn-DsRed2N1-EGFP-siRNA vector (V009658)

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LOCUS       40924_3271        6990 bp DNA     circular SYN 17-DEC-2018
DEFINITION  SiRNA vector pAAV9(5)-hSyn-DsRed2N1-EGFP-siRNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Michel U, Malik I, Ebert S, Bahr M, Kugler S.
  TITLE     Long-term in vivo and in vitro AAV-2-mediated RNA interference in 
            rat retinal ganglion cells and cultured primary neurons
  JOURNAL   Biochem. Biophys. Res. Commun. 326 (2), 307-312 (2005)
  PUBMED    15582578
REFERENCE   2  (bases 1 to 6990)
  AUTHORS   Michel U, Malik I, Kuegler S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2004) Neurology, University of Goettingen, Waldweg
            33, Goettingen 37073, Germany
REFERENCE   3  (bases 1 to 6990)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6990)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2005"; volume: "326"; issue: "2"; 
            pages: "307-312"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2004) Neurology, University of Goettingen, Waldweg 33, 
            Goettingen 37073, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(51..185)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(201..334)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(351..1025)
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     misc_feature    complement(1044..1062)
                     /note="MCS; multiple cloning site"
     promoter        complement(1068..1515)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     promoter        1543..1757
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     misc_feature    1759..1822
                     /note="siEGFP; 64 bp siRNA sequence"
     gene            complement(1827..4090)
                     /gene="9(5)"
                     /label=9(5)
                     /note="partial sequence from the non-coding porcine RNA UM
                     9(5)"
     repeat_region   4110..4250
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4325..4780
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5062..5166
                     /label=AmpR promoter
     CDS             5167..6024
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6198..6786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     repeat_region   6848..6988
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"