pAAV9(5)-CMV-EGFP-CytB-AS-ohneNot vector (V009659)

LOCUS       40924_3266        7187 bp DNA     circular SYN 17-DEC-2018
DEFINITION  SiRNA vector pAAV9(5)-CMV-EGFP-CytB-AS-ohneNot, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Michel U, Malik I, Ebert S, Bahr M, Kugler S.
  TITLE     Long-term in vivo and in vitro AAV-2-mediated RNA interference in 
            rat retinal ganglion cells and cultured primary neurons
  JOURNAL   Biochem. Biophys. Res. Commun. 326 (2), 307-312 (2005)
  PUBMED    15582578
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Michel U, Malik I, Kuegler S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2004) Neurology, University of Goettingen, Waldweg
            33, Goettingen 37073, Germany
REFERENCE   3  (bases 1 to 7187)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7187)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2005"; volume: "326"; issue: "2"; 
            pages: "307-312"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2004) Neurology, University of Goettingen, Waldweg 33, 
            Goettingen 37073, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     polyA_signal    complement(194..328)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(344..477)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(492..1208)
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    complement(1227..1246)
                     /note="MCS; multiple cloning site"
     promoter        complement(1249..1777)
                     /label=mCMV promoter
                     /note="mouse cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     1779..1795
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1815..2029
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     misc_feature    2031..2162
                     /note="Cytb-AS; partial human cytochrome B antisense
                     sequence"
     gene            complement(2167..4430)
                     /gene="9(5)"
                     /label=9(5)
                     /note="partial sequence from the non-coding porcine RNA UM
                     9(5)"
     repeat_region   4450..4590
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4665..5120
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5402..5506
                     /label=AmpR promoter
     CDS             5507..6364
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6538..7126
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"