pAAV2R585E-SBBXEB-VBC vector (V009661)

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LOCUS       V009661                 7934 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009661
VERSION     V009661
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Adachi K, Enoki T, Kawano Y, Veraz M, Nakai H.
  TITLE     Drawing a high-resolution functional map of adeno-associated virus
            capsid by massively parallel sequencing
  JOURNAL   Nat Commun 5, 3075 (2014)
   PUBMED   24435020
REFERENCE   2  (bases 1 to 7934)
  AUTHORS   Nakai H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2013) Department of Molecular and Medical
            Genetics, Oregon Health and Science University, 3181 SW Sam Jackson
            Park Road, Portland, OR 97239, USA
REFERENCE   3  (bases 1 to 7934)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7934)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun
            5, 3075 (2014)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-MAY-2013) Department of Molecular and Medical Genetics, Oregon
            Health and Science University, 3181 SW Sam Jackson Park Road,
            Portland, OR 97239, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     CDS             317..2179
                     /gene="Rep78"
                     /label="Protein Rep78"
                     /note="Protein Rep78 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89268"
     CDS             2199..4403
                     /gene="VP1"
                     /label="Capsid protein VP1"
                     /note="Capsid protein VP1 from Adeno-associated virus 2
                     (isolate Srivastava/1982). Accession#: P03135"
     gap             4528..4539
                     /estimated_length=12
     gap             4560..4571
                     /estimated_length=12
     repeat_region   4677..4817
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     primer_bind     complement(4827..4843)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5056..5511
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5793..5897
                     /label="AmpR promoter"
     CDS             5898..6755
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6929..7517
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7805..7826
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7841..7871
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7879..7895
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7903..7919
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"