pAAV-EF1alpha-hFAH.AOS2 vector (V009672)

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LOCUS       V009672                 6772 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009672
VERSION     V009672
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Nakai H, Wu X, Fuess S, Storm TA, Munroe D, Montini E, Burgess SM,
            Grompe M, Kay MA.
  TITLE     Large-scale molecular characterization of adeno-associated virus
            vector integration in mouse liver
  JOURNAL   J. Virol. 79 (6), 3606-3614 (2005)
   PUBMED   15731255
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Nakai H, Wu X, Fuess S, Storm TA, Munroe D, Montini E, Burgess SM,
            Grompe M, Kay MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JAN-2007) Department of Molecular Genetics and
            Biochemistry, University of Pittsburgh School of Medicine, W1244 BST
            200 Lothrop Street, Pittsburgh, PA 15261, USA
REFERENCE   3  (bases 1 to 6772)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6772)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "2005"; volume: "79"; issue: "6"; pages: "3606-3614"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JAN-2007) Department of Molecular Genetics and Biochemistry,
            University of Pittsburgh School of Medicine, W1244 BST 200 Lothrop
            Street, Pittsburgh, PA 15261, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          233..1175
                     /label="EF-1-alpha intron A"
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
     CDS             1243..2499
                     /gene="FAH"
                     /label="Fumarylacetoacetase"
                     /note="Fumarylacetoacetase from Homo sapiens. Accession#:
                     P16930"
     regulatory      2625..2834
                     /label="poly A signal from Homo sapiens beta globin"
                     /note="poly A signal from Homo sapiens beta globin"
                     /regulatory_class="polyA_signal_sequence"
     promoter        2878..2982
                     /label="AmpR promoter"
     CDS             2983..3840
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4014..4602
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     repeat_region   4734..4863
                     /note="adeno-associated virus serotype 2 inverted terminal
                     repeat"
     repeat_region   6643..6772
                     /note="adeno-associated virus serotype 2 inverted terminal
                     repeat"