pAAV-9(5)-CMV-EGFP-CytbAS vector (V009687)

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LOCUS       40924_3007        7187 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-9(5)-CMV-EGFP-CytbAS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Michel U, Muller B, Koch JC, Zhang J-N.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2015) Neurology, University of Goettingen, Waldweg
            33, Goettingen, Niedersachsen 37073, Germany
REFERENCE   2  (bases 1 to 7187)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7187)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (27-JUL-2015) Neurology, University of Goettingen, Waldweg 33, 
            Goettingen, Niedersachsen 37073, Germany"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(40..174)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(190..323)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(338..1054)
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(1095..1623)
                     /label=mCMV promoter
                     /note="mouse cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     1625..1641
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1661..1875
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     repeat_region   4296..4436
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4511..4966
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5248..5352
                     /label=AmpR promoter
     CDS             5353..6210
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6384..6972
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     repeat_region   7034..7174
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"