pAA3.7X vector (V009689)

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LOCUS       V009689                 9583 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009689
VERSION     V009689
KEYWORDS    complete genome
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9583)
  AUTHORS   Ahmed A.
  TITLE     A rapid procedure for DNA sequencing using transposon-promoted
            deletions in Escherichia coli
  JOURNAL   Gene 39 (2-3), 305-310 (1985)
   PUBMED   3005115
REFERENCE   2  (bases 1 to 9583)
  AUTHORS   Ahmed A.
  TITLE     Sequencing with transposon-promoted deletions: Use of
            transposon-promoted deletions in DNA sequence analysis
  JOURNAL   (in) Wu,R. and Grossman,L. (Eds.); METHODS IN ENZYMOLOGY,
            RECOMBINANT DNA, PART E; Academic Press, New York (1986)
REFERENCE   3  (bases 1 to 9583)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9583)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 39
            (2-3), 305-310 (1985)"
            SGRef: number: 2; type: "Journal Article"; journalName: "(in) Wu,R.
            and Grossman,L. (Eds.); METHODS IN ENZYMOLOGY, RECOMBINANT DNA, PART
            E; Academic Press, New York (1986)"
            SGRef: number: 3; type: "Journal Article"
            [2]  revises [1].
            Sequence in computer readable form and reprints for [1],[(in) Wu,R.
            and Grossman,L., (Eds.);Methods in Enzymology, Recombinant DNA, part
            ] and [2]
            kindly provided by A.Ahmed, 31-MAR-1986.
            [1] describes a new quick sequencing method, which involves the
            subdivision of the DNA fragment to be sequenced into many
            overlapping segments by a series of deletions which start from a
            fixed site.  Since the different segments retain their natural
            connectedness, the complete sequence is easily assembled from
            overlaps that occur between the sequences of adjacent deletions.  A
            simple and rapid method for the isolation of transposon-promoted
            deletions and their use in sequencing long DNA fragments was
            developed.
FEATURES             Location/Qualifiers
     source          1..9583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(79..1224)
                     /gene="galK"
                     /label="Galactokinase"
                     /note="Galactokinase from Escherichia coli O157:H7 (strain
                     EC4115 / EHEC). Accession#: B5YRF5"
     CDS             2927..4114
                     /label="TcR"
                     /note="tetracycline efflux protein"
     CDS             4758..4946
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    5051..5191
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5377..5965)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6139..6996)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6997..7101)
                     /label="AmpR promoter"
     mobile_element  complement(7122..7889)
                     /label="IS1"
                     /note="prokaryotic transposable element"
     promoter        8010..8112
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             8113..8769
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"