pA1.GFP.A2 vector (V009691)

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LOCUS       40924_2982       12653 bp DNA     circular SYN 17-DEC-2018
DEFINITION  AAVS1 targeting vector pA1.GFP.A2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12653)
  AUTHORS   van Nierop GP, de Vries AA, Holkers M, Vrijsen KR, Goncalves MA.
  TITLE     Stimulation of homology-directed gene targeting at an endogenous 
            human locus by a nicking endonuclease
  JOURNAL   Nucleic Acids Res. 37 (17), 5725-5736 (2009)
  PUBMED    19651880
REFERENCE   2  (bases 1 to 12653)
  AUTHORS   van Nierop GP, de Vries AA, Holkers M, Vrijsen KR, Goncalves MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2009) Molecular Cell Biology, Leiden University 
            Medical Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The 
            Netherlands
REFERENCE   3  (bases 1 to 12653)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12653)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2009"; volume: "37"; issue: "17"; pages: 
            "5725-5736"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JUL-2009) Molecular Cell Biology, Leiden University Medical 
            Center, Einthovenweg 20, Leiden, Zuid Holland 2333 ZC, The 
            Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1216..2397
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     LTR             2505..2773
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(2810..2828)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2895..3611
                     /label=hrGFP
                     /note="humanized Renilla green fluorescent protein"
     polyA_signal    complement(3646..3767)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    4062..4563
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    4685..5521
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     rep_origin      complement(8629..9017)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     oriT            complement(9035..9325)
                     /direction=LEFT
                     /label=oriT
                     /note="origin of transfer for the bacterial F plasmid
                     (Frost et al., 1994)"
     CDS             complement(9381..10172)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    10588..12626
                     /note="Arm 1; human chromosome 19 AAVS1 locus region"