p713-1555 vector (V009706)

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LOCUS       40924_2932        3341 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p713-1555, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Shigaki T, Kole M, Hirschi KD.
  TITLE     Cre-loxP recombination vectors for the expression of Riken 
            full-length plant cDNAs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Shigaki T, Kole M, Hirschi KD.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2005) Children's Nutrition Research Center, Baylor
            College of Medicine, Room 9016, 1100 Bates St., Houston, TX 77030, 
            USA
REFERENCE   3  (bases 1 to 3341)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3341)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-MAY-2005) Children's Nutrition Research Center, Baylor College 
            of Medicine, Room 9016, 1100 Bates St., Houston, TX 77030, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(127..160)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    183..903
                     /note="stuffer DNA from Arabidopsis thaliana cation/proton 
                     exchanger CAX2"
     terminator      922..1174
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      1247..1294
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(1390..1778)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     rep_origin      complement(1793..2248)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(2285..3142)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3143..3247)
                     /label=AmpR promoter