p5xATF6 GL3 vector (V009709)

LOCUS       40924_2917        5065 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector p5xATF6 GL3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Wang Y, Shen J, Arenzana N, Tirasophon W, Kaufman RJ, Prywes R.
  TITLE     Activation of ATF6 and an ATF6 DNA binding site by the endoplasmic 
            reticulum stress response
  JOURNAL   J. Biol. Chem. 275 (35), 27013-27020 (2000)
  PUBMED    10856300
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Shen J, Wang Y, Prywes R.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2005) Biological Sciences, Columbia University, 
            New York, NY 10027, USA
REFERENCE   3  (bases 1 to 5065)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5065)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Biol. 
            Chem."; date: "2000"; volume: "275"; issue: "35"; pages: 
            "27013-27020"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-JUN-2005) Biological Sciences, Columbia University, New York, NY
            10027, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      205..308
                     /label=c-fos minimal promoter
                     /note="c-fos minimal promoter"
                     /regulatory_class="promoter"
     CDS             335..1984
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(2028..2149)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2568..3156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3330..4187)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4188..4292)
                     /label=AmpR promoter
     rep_origin      4319..4774
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4905..4953
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4967..5058
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"