p413-SSRE-GFP vector (V009729)

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LOCUS       40924_2757        6894 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector p413-SSRE-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Chen M-T., Weiss R.
  TITLE     Artificial cell-cell communication in yeast Saccharomyces cerevisiae
            using signaling elements from Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Chen M-T., Weiss R.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2005) Electrical Engineering, Princeton 
            University, Engineering Quadrangle, Olden Street, Princeton, NJ 
            08544, USA
REFERENCE   3  (bases 1 to 6894)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6894)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-OCT-2005) Electrical Engineering, Princeton University, 
            Engineering Quadrangle, Olden Street, Princeton, NJ 08544, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        317..503
                     /label=HIS3 promoter
     CDS             504..1160
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     rep_origin      complement(1423..1878)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2023..2039
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2049..2067
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(2438..2625)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(2649..3362)
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      complement(3382..4074)
                     /label=SSRE promoter
                     /note="SSRE promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(4084..4100)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4120..4138)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4159..4175)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4183..4199)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4207..4237)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4252..4273)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4561..5149)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5323..6180)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6181..6285)
                     /label=AmpR promoter
     misc_feature    6322..6825
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"