p35S-loxP-BSD vector (V009749)

LOCUS       40924_2627        4876 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Moss transformation vector p35S-loxP-BSD DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Aoyama T, Hiwatashi Y, Hasebe M.
  TITLE     Moss transformation vector p35S-loxP-BSD
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Aoyama T, Hiwatashi Y, Hasebe M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2009) Contact:Yuji Hiwatashi National Institute 
            for Basic Biology, Evolutionary Biology; Myodaiji, Nishigonaka, 38, 
            Okazaki, Aichi 444-8585, Japan
REFERENCE   3  (bases 1 to 4876)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4876)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-DEC-2009) Contact:Yuji Hiwatashi National Institute for Basic 
            Biology, Evolutionary Biology; Myodaiji, Nishigonaka, 38, Okazaki, 
            Aichi 444-8585, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     708..741
                     /label=loxP
                     /note="loxP"
     protein_bind    708..741
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        884..1229
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1268..1654
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="PLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVNV
                     YHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKAI
                     VKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    1800..1976
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    complement(2563..2596)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2688..2706)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2727..2743)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2751..2767)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2775..2805)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2820..2841)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3129..3717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3891..4748)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4749..4853)
                     /label=AmpR promoter