p1cVTm vector (V009779)

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LOCUS       40924_2448        4013 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p1cVTm, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Boehm CR, Grant PK, Haseloff J.
  TITLE     Programmed hierarchical patterning of bacterial populations
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Boehm CR, Grant PK, Haseloff J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2017) Plant Sciences, University of Cambridge, 
            Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom
REFERENCE   3  (bases 1 to 4013)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4013)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-FEB-2017) Plant Sciences, University of Cambridge, Downing 
            Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(49..637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      818..852
                     /label=PJ23101*
                     /note="PJ23101*"
                     /regulatory_class="promoter"
     regulatory      861..878
                     /label=BBa_B0034
                     /note="BBa_B0034"
                     /regulatory_class="ribosome_binding_site"
     RBS             861..872
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             879..1595
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
     terminator      1618..1689
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1705..1732
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1748..2605)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2606..2710)
                     /label=AmpR promoter
     terminator      complement(2800..2843)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2846..2867
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     terminator      complement(2874..2901)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(2917..2988)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(3011..3727)
                     /label=mTurquoise2
                     /note="enhanced monomeric variant of CFP (Goedhart et al., 
                     2012)"
     regulatory      complement(3728..3745)
                     /label=BBa_B0034
                     /note="BBa_B0034"
                     /regulatory_class="ribosome_binding_site"
     RBS             3734..3745
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      complement(3754..3788)
                     /label=PJ23101
                     /note="PJ23101"
                     /regulatory_class="promoter"
     misc_feature    3789..3809
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      3810..3867
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."