p119L1e vector (V009785)

Basic Vector Information

Vector Name:
p119L1e
Antibiotic Resistance:
Kanamycin
Length:
8290 bp
Type:
Cloning vector
Replication origin:
ori
Source/Author:
Maclean JM, Williamson A-L., Jacobs WR.

p119L1e vector Vector Map

p119L1e8290 bp400800120016002000240028003200360040004400480052005600600064006800720076008000KanRoriThymidylate kinaseMajor capsid protein L1rrnB T1 terminator

p119L1e vector Sequence

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LOCUS       V009785                 8290 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V009785
VERSION     V009785
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Maclean JM, Williamson A-L., Jacobs WR.
  TITLE     Investigation of the use of recombinant BCG, expressing the major
            capsid protein (L1) of human papillomavirus type 16, as a candidate
            vaccine for cervical cancer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Maclean JM, Williamson A-L., Jacobs WR.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2005) Division of Medical Virology, Department of
            Clinical Laboratory Science and Institute of Infectious Diseases and
            Molecular Medicine, University of Cape Town, Rondebosch, Cape Town
            7701, South Africa
REFERENCE   3  (bases 1 to 8290)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8290)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-MAR-2005) Division of Medical Virology, Department of Clinical
            Laboratory Science and Institute of Infectious Diseases and
            Molecular Medicine, University of Cape Town, Rondebosch, Cape Town
            7701, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1877..2689
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             5698..6330
                     /gene="tmk"
                     /label="Thymidylate kinase"
                     /note="Thymidylate kinase from Mycobacterium tuberculosis
                     (strain ATCC 25618 / H37Rv). Accession#: P9WKE1"
     CDS             6571..8085
                     /gene="L1"
                     /label="Major capsid protein L1"
                     /note="Major capsid protein L1 from Human papillomavirus
                     type 16. Accession#: P03101"
     terminator      8208..8254
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"

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