p(T5CkB)3-50huIL6P-luc+ vector (V009813)

LOCUS       40924_2318        4971 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector p(T5CkB)3-50huIL6P-luc+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4971)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4971)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      78..95
                     /label=T5CkB
                     /note="T5CkB"
                     /regulatory_class="promoter"
     regulatory      96..113
                     /label=T5CkB
                     /note="T5CkB"
                     /regulatory_class="promoter"
     regulatory      114..131
                     /label=T5CkB
                     /note="T5CkB"
                     /regulatory_class="promoter"
     regulatory      144..193
                     /label=hIL6f
                     /note="hIL6f"
                     /regulatory_class="promoter"
     CDS             241..1890
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(1934..2055)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2474..3062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3236..4093)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4094..4198)
                     /label=AmpR promoter
     rep_origin      4225..4680
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4811..4859
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4873..4964
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"