OX4389 vector (V009818)

LOCUS       40924_2293        7990 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector OX4389, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Martins S, Naish N, Walker AS, Morrison NI, Scaife S, Fu G, 
            Dafa'alla T, Alphey L.
  TITLE     Germline transformation of the diamondback moth, Plutella xylostella
            L., using the piggyBac transposable element
  JOURNAL   Insect Mol. Biol. 21 (4), 414-421 (2012)
  PUBMED    22621377
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Martins S, Naish N, Walker A, Morrison N, Scaife S, Fu G, Dafa'alla 
            T, Luke A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2012) Research, Oxitec Ltd, 71 Milton Park, 
            Abingdon, Oxon OX14 4RX, UK
REFERENCE   3  (bases 1 to 7990)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7990)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Insect Mol.
            Biol."; date: "2012"; volume: "21"; issue: "4"; pages: "414-421"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JAN-2012) Research, Oxitec Ltd, 71 Milton Park, Abingdon, Oxon 
            OX14 4RX, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..1426
                     /mobile_element_type="transposon:piggyBac 5'"
                     /label=5'
     polyA_signal    complement(1467..1588)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(1704..1768)
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(1769..2461)
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
     misc_feature    complement(2495..2521)
                     /label=nuclear localization signal
                     /note="nuclear localization signal"
     regulatory      complement(2525..3065)
                     /label=Opie2
                     /note="Opie2"
                     /regulatory_class="promoter"
     protein_bind    3544..3643
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     mobile_element  3718..4494
                     /mobile_element_type="transposon:piggyBac 3'"
                     /label=3'
     primer_bind     complement(5195..5211)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5685..5789
                     /label=AmpR promoter
     CDS             5790..6647
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6821..7409
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7697..7718
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7733..7763
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7771..7787
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7795..7811
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"