nvYFP w- vector (V009829)

LOCUS       40924_2259        7641 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector nvYFP w-, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Le T, Liang Z, Patel H, Yu MH, Sivasubramaniam G, Slovitt M, 
            Tanentzapf G, Mohanty N, Paul SM, Wu VM, Beitel GJ.
  TITLE     A new family of Drosophila balancer chromosomes with a w- dfd-GMR 
            yellow fluorescent protein marker
  JOURNAL   Genetics 174 (4), 2255-2257 (2006)
  PUBMED    17057238
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Le T, Liang Z, Beitel GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2006) Biochemistry, Molecular Biology and Cell 
            Biology, Northwestern University, 2205 Tech Drive, Evanston, IL 
            60208, USA
REFERENCE   3  (bases 1 to 7641)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7641)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2006"; volume: "174"; issue: "4"; pages: "2255-2257"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JUN-2006) Biochemistry, Molecular Biology and Cell Biology, 
            Northwestern University, 2205 Tech Drive, Evanston, IL 60208, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      79..2813
                     /label=dfd HZ2.7 promoter
                     /note="dfd HZ2.7 promoter"
                     /regulatory_class="promoter"
     regulatory      2819..2999
                     /label=GMR enhancer
                     /note="GMR enhancer"
                     /regulatory_class="enhancer"
     CDS             3204..3920
                     /label=mVenus
                     /note="Venus YFP with monomerizing A206K mutation (Nagai et
                     al., 2002; Kremers et al., 2006)"
     polyA_signal    4196..4317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4882..5470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5644..6501)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6502..6606)
                     /label=AmpR promoter
     misc_feature    complement(7409..7641)
                     /label=P element 3' end
                     /note="P element 3' end"