nLucFlucGW vector (V009833)

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LOCUS       40924_2239        9789 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector nLucFlucGW, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9789)
  AUTHORS   Reis RS.
  TITLE     nLuc/Fluc dual luciferase for protoplast transformation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9789)
  AUTHORS   Reis RS.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2018) Department of Plant Molecular Biology, 
            University of Lausanne, Quartier UNIL-Sorge, Batiment Biophore, 
            Lausanne, Vaud 1015, Switzerland
REFERENCE   3  (bases 1 to 9789)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9789)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-JUN-2018) Department of Plant Molecular Biology, University of 
            Lausanne, Quartier UNIL-Sorge, Batiment Biophore, Lausanne, Vaud 
            1015, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     regulatory      324..1449
                     /label=RHIP1pro
                     /note="RHIP1pro"
                     /regulatory_class="promoter"
     protein_bind    1470..1594
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1619..1649
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1703..2359
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2704..3006
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3050..3174)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3188..3253
                     /label=F2A
                     /note="2A peptide from foot-and-mouth disease virus
                     polyprotein"
     CDS             3272..3784
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     CDS             3785..3811
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     terminator      3834..4082
                     /label=HSP terminator
                     /note="efficient transcription terminator from the
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     protein_bind    complement(4091..4115)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5850..7499
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      7529..7781
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        8118..8188
                     /label=AmpR promoter
     CDS             8189..9046
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      9139..9727
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"