mceG vector (V009852)

LOCUS       V009852                 5871 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009852
VERSION     V009852
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Joshi SM, Pandey AK, Capite N, Fortune SM, Rubin EJ, Sassetti CM.
  TITLE     Characterization of mycobacterial virulence genes through genetic
            interaction mapping
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 103 (31), 11760-11765 (2006)
   PUBMED   16868085
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Joshi SM, Pandey AK, Capite NM, Fortune SM, Rubin EJ, Sassetti CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2006) Molecular Genetics and Microbiology,
            University of Massachusetts Medical School, 55 Lake Ave. North,
            Worcester, MA 01655, USA
REFERENCE   3  (bases 1 to 5871)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5871)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2006"; volume: "103"; issue: "31"; pages:
            "11760-11765"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-JUN-2006) Molecular Genetics and Microbiology, University of
            Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA
            01655, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..394
                     /note="promoter from Mycobacterium tuberculosis groEL2
                     gene"
                     /regulatory_class="promoter"
     CDS             443..1519
                     /gene="mkl"
                     /label="Probable ribonucleotide transport ATP-binding
                     protein mkl"
                     /note="Probable ribonucleotide transport ATP-binding
                     protein mkl from Mycobacterium tuberculosis (strain ATCC
                     25618 / H37Rv). Accession#: P9WQL5"
     CDS             1520..1537
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             1544..1573
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             1931..2695
                     /label="ApmR"
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     rep_origin      3204..3792
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      3951..5845
                     /label="mycobacterial origin of replication from pAL5000"
                     /note="mycobacterial origin of replication from pAL5000"