LMP-1 vector (V009861)

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LOCUS       V009861                 5527 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009861
VERSION     V009861
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Lee J, Sugden B.
  TITLE     A membrane leucine heptad contributes to trafficking, signaling, and
            transformation by latent membrane protein 1
  JOURNAL   J. Virol. 81 (17), 9121-9130 (2007)
   PUBMED   17581993
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Lee J, Sugden B.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2010) Department of Oncology, McArdle Laboratory
            for Cancer Research, University of Wisconsin-Madison, 1400
            University Ave, Madison, WI 53705, USA
REFERENCE   3  (bases 1 to 5527)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5527)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "2007"; volume: "81"; issue: "17"; pages: "9121-9130"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-DEC-2010) Department of Oncology, McArdle Laboratory for Cancer
            Research, University of Wisconsin-Madison, 1400 University Ave,
            Madison, WI 53705, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..366
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     intron          395..967
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     promoter        1022..1040
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1066..2223
                     /gene="LMP1"
                     /label="Latent membrane protein 1"
                     /note="Latent membrane protein 1 from Epstein-Barr virus
                     (strain B95-8). Accession#: P03230"
     polyA_signal    2523..2644
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2849..3437)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3611..4468)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4469..4573)
                     /label="AmpR promoter"
     rep_origin      complement(4906..5361)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5506..5522
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"