Basic Vector Information
- Vector Name:
- LIC-pDEST-LC2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6349 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Dortay H, Akula UM, Westphal C, Sittig M, Mueller-Roeber B.
- Promoter:
- tet
LIC-pDEST-LC2 vector Map
LIC-pDEST-LC2 vector Sequence
LOCUS 40924_1754 6349 bp DNA circular SYN 17-DEC-2018 DEFINITION Expression vector LIC-pDEST-LC2, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6349) AUTHORS Dortay H, Akula UM, Westphal C, Sittig M, Mueller-Roeber B. TITLE High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection JOURNAL PLoS ONE 6 (4), E18900 (2011) PUBMED 21541323 REFERENCE 2 (bases 1 to 6349) AUTHORS Dortay H, Mueller-Roeber B. TITLE Direct Submission JOURNAL Submitted (12-FEB-2011) Molecular Biology, University of Potsdam, Germany, Karl-Liebknecht-Str. 24-25, Haus 20, Potsdam, Brandenburg 14476, Germany REFERENCE 3 (bases 1 to 6349) TITLE Direct Submission REFERENCE 4 (bases 1 to 6349) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; date: "2011"; volume: "6"; issue: "4"; pages: "E18900" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (12-FEB-2011) Molecular Biology, University of Potsdam, Germany, Karl-Liebknecht-Str. 24-25, Haus 20, Potsdam, Brandenburg 14476, Germany" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6349 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 10..17 /label=PmeI recognition site /note="PmeI recognition site" misc_feature 18..684 /label=LIC stuffer fragment /note="LIC stuffer fragment" misc_feature 685..692 /label=PmeI recognition site /note="PmeI recognition site" CDS 698..718 /codon_start=1 /label=TEV site /note="tobacco etch virus (TEV) protease recognition and cleavage site" /translation="ENLYFQG" CDS 728..1711 /codon_start=1 /label=IFP1.4 /note="bacteriophytochrome-based monomeric infrared fluorescent protein (Shu et al., 2009)" /translation="MARDPLPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPEQWPALQAALPPGCPDALQYRATLD WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT VRELTGFDRVMLYKFAPDATGEMIAEARREGMQAFLGHRFPASHTPAQARALYTRHLLR LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG QLWGLIVCHHQTPYVLPPDLRTTLEELGRKLSGQVQRKEAGMDELYK" CDS 1718..1735 /codon_start=1 /label=6xHis /note="6xHis affinity tag" /translation="HHHHHH" misc_feature 1736..1738 /label=stop codon (TAG) /note="stop codon (TAG)" terminator 1803..1850 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" promoter complement(2216..2244) /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" promoter 2357..2461 /label=AmpR promoter CDS 2462..3319 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 3493..4081 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(4267..4409) /label=bom /note="basis of mobility region from pBR322" CDS complement(4514..4702) /codon_start=1 /label=rop /note="Rop protein, which maintains plasmids at low copy number" /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" promoter 6267..6285 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" RBS 6317..6339 /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" misc_feature 6347..6349 /label=start codon /note="start codon"
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