JSC vector (V009903)

LOCUS       40924_1444        4566 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector JSC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Sepulveda J, Shoemaker CB.
  TITLE     Design and testing of PCR primers for the construction of scFv 
            libraries representing the immunoglobulin repertoire of rats
  JOURNAL   J. Immunol. Methods 332 (1-2), 92-102 (2008)
  PUBMED    18242637
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Shoemaker CB, Sepulveda J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2007) Biomedical Science, Tufts Cummings School of
            Veterinary Medicine, 200 Westboro Rd., North Grafton, MA 01536, USA
REFERENCE   3  (bases 1 to 4566)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4566)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Immunol.
            Methods 332 (1-2), 92-102 (2008)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-AUG-2007) Biomedical Science, Tufts Cummings School of 
            Veterinary Medicine, 200 Westboro Rd., North Grafton, MA 01536, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    330..351
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        366..396
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    404..420
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     sig_peptide     443..508
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     misc_feature    508..593
                     /label=multi-cloning site
                     /note="multi-cloning site"
     misc_feature    593..631
                     /label=E-tag
                     /note="E-tag"
     CDS             641..1852
                     /codon_start=1
                     /label=M13 gene III
                     /note="pIII"
                     /translation="TVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVVVCT
                     GDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTYINPL
                     DGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTDPVKTY
                     YQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAGGGSGGG
                     SGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENADENALQSD
                     AKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDNSPLMNNFR
                     QYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMYVFSTFANIL
                     RNKES"
     primer_bind     complement(1862..1878)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2091..2546
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2828..2932
                     /label=AmpR promoter
     CDS             2933..3790
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3964..4552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"