ipSilent vector (V009905)

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LOCUS       40924_1414        6086 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gene silencing vector ipSilent DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Vu BV, Pham KT, Nakayashiki H.
  TITLE     Substrate-Induced Transcriptional Activation of the MoCel7C 
            Cellulase Gene Is Associated with Methylation of Histone H3 at 
            Lysine 4 in the Rice Blast Fungus Magnaporthe oryzae
  JOURNAL   Appl. Environ. Microbiol. 79 (21), 6823-6832 (2013)
  PUBMED    23995923
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   Nakayashiki H, Vu BV.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2012) Contact:Hitoshi Nakayashiki Kobe University,
            Faculty of Agriculture; 1-1 Rokkodai, Nada, Kobe, Hyogo 657-8501, 
            Japan
REFERENCE   3  (bases 1 to 6086)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6086)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2013"; volume: "79"; issue: "21"; 
            pages: "6823-6832"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-SEP-2012) Contact:Hitoshi Nakayashiki Kobe University, Faculty 
            of Agriculture; 1-1 Rokkodai, Nada, Kobe, Hyogo 657-8501, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2234..2588
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             2593..3615
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     regulatory      3619..3864
                     /label=Aspergillus nidulans TrpC terminator
                     /note="Aspergillus nidulans TrpC terminator"
                     /regulatory_class="terminator"
     primer_bind     3873..3889
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      3892..4990
                     /label=Magnaporthe oryzae MoCel7C promoter
                     /note="Magnaporthe oryzae MoCel7C promoter"
                     /regulatory_class="promoter"
     misc_feature    4991..5028
                     /label=multi-cloning sites
                     /note="multi-cloning sites"
     intron          5029..5162
                     /note="Magnaporthe oryzae cutinase intron2"
     misc_feature    5163..5228
                     /label=multi-cloning sites
                     /note="multi-cloning sites"
     regulatory      5229..5432
                     /label=Magnaporthe oryzae MoCel7C terminator
                     /note="Magnaporthe oryzae MoCel7C terminator"
                     /regulatory_class="terminator"
     promoter        complement(5443..5461)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5471..5487)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5629..6084
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"