HSP18.2-LUC-NOS-At5g59720 vector (V009916)

LOCUS       40924_1344        5452 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Control vector HSP18.2-LUC-NOS-At5g59720, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Kovtun Y, Chiu WL, Tena G, Sheen J.
  TITLE     Functional analysis of oxidative stress-activated mitogen-activated 
            protein kinase cascade in plants
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (6), 2940-2945 (2000)
  PUBMED    10717008
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Sheen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) Molecular Biology, Massachusetts General 
            Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, 
            Boston, MA 02114, USA
REFERENCE   3  (bases 1 to 5452)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5452)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2000"; volume: "97"; issue: "6"; pages: 
            "2940-2945"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185
            Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..31)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(39..55)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(63..93)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(108..129)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(417..1005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1179..2036)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2037..2141)
                     /label=AmpR promoter
     primer_bind     2615..2631
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2652..2668
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2670..3388
                     /label=Hsp18.2 enhancer/promoter
                     /note="Hsp18.2 enhancer/promoter"
                     /regulatory_class="enhancer"
     CDS             3389..5038
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      5196..5447
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"