Basic Vector Information
- Vector Name:
- GAANTRY B donor tdTom
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8923 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Collier R, Thomson JG, Thilmony R.
- Promoter:
- CaMV35S(enhanced)
GAANTRY B donor tdTom vector Map
GAANTRY B donor tdTom vector Sequence
LOCUS 40924_959 8923 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector GAANTRY B donor tdTom, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8923) AUTHORS Collier R, Thomson JG, Thilmony R. TITLE GAANTRY, a versatile and robust Agrobacterium-based gene stacking system generates high quality transgenic plants JOURNAL Unpublished REFERENCE 2 (bases 1 to 8923) AUTHORS Thilmony R. TITLE Direct Submission JOURNAL Submitted (15-DEC-2017) Crop Improvement and Genetics research Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA REFERENCE 3 (bases 1 to 8923) TITLE Direct Submission REFERENCE 4 (bases 1 to 8923) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (15-DEC-2017) Crop Improvement and Genetics research Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..8923 /mol_type="other DNA" /organism="synthetic DNA construct" polyA_signal complement(38..212) /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" regulatory complement(218..446) /label=nos terminator /note="nos terminator" /regulatory_class="terminator" CDS complement(490..1917) /label=tdTomato /note="tandem dimeric (pseudo-monomeric) derivative of DsRed (Shaner et al., 2004)" promoter complement(2032..2708) /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" misc_feature 2937..2991 /label=TP901 attB /note="TP901 attB" misc_feature 3013..3037 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" promoter 3196..3224 /label=Pc promoter /note="class 1 integron promoter" CDS 3413..3943 /label=GmR /note="gentamycin acetyltransferase" misc_feature 4022..4127 /label=ParA MRS /note="ParA MRS" primer_bind complement(4150..4166) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(4174..4190) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4198..4228) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4243..4264) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 4497..4942 /label=sacB promoter /note="sacB promoter and control region" CDS 4943..6361 /label=SacB /note="secreted levansucrase that renders bacterial growth sensitive to sucrose" rep_origin complement(6462..7050) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7224..8081) /label=AmpR /note="beta-lactamase" promoter complement(8082..8186) /label=AmpR promoter rep_origin complement(8212..8667) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind 8809..8825 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 8856..8911 /label=A118 attB /note="A118 attB"
This page is informational only.