FMDV-2A-def vector (V009984)

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LOCUS       V009984                14325 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009984
VERSION     V009984
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14325)
  AUTHORS   Shustov AV, Frolov IV.
  TITLE     Trans-packaging of Yellow Fever virus replicons: an approach for
            flaviviral antigen production
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14325)
  AUTHORS   Shustov AV, Frolov IV.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2005) Department of Microbiology and Immunology,
            University of Texas Medical Branch, 301 University Boulevard,
            Galveston, TX 77550-1019, USA
REFERENCE   3  (bases 1 to 14325)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14325)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-DEC-2005) Department of Microbiology and Immunology, University
            of Texas Medical Branch, 301 University Boulevard, Galveston, TX
            77550-1019, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2..197
                     /mol_type="other DNA"
                     /db_xref="taxon:11090"
                     /organism="Yellow fever virus 17D"
     misc_feature    2..119
                     /label="5'UTR of Yellow fever virus 17D"
                     /note="5'UTR of Yellow fever virus 17D"
     misc_feature    120..194
                     /note="first 25 aa of Yellow fever virus 17D capsid
                     protein"
     CDS             198..914
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    918..968
                     /label="2A protease of Foot-and-Mouth Disease Virus"
                     /note="2A protease of Foot-and-Mouth Disease Virus"
     CDS             969..10901
                     /note="Genome polyprotein from Yellow fever virus (strain
                     17D vaccine). Accession#: P03314"
                     /label="Genome polyprotein"
     misc_feature    10905..11412
                     /label="3'UTR of Yellow fever virus 17D"
                     /note="3'UTR of Yellow fever virus 17D"
     polyA_signal    11622..11703
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(12367..12911)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(13079..13936)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(13937..14041)
                     /label="AmpR promoter"