cp9 vector (V010034)

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LOCUS       40924_510        5473 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector cp9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Elias AF, Bono JL, Kupko JJ III, Stewart PE, Krum JG, Rosa P.
  TITLE     New antibiotic resistance cassettes suitable for genetic studies in 
            Borrelia burgdorferi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Krum JG, Bono JL, Elias AF, Rosa P.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2002) LHBP, RML, NIAID, NIH, 903 South 4th Street,
            Hamilton, MT 59840, USA
REFERENCE   3  (bases 1 to 5473)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5473)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-NOV-2002) LHBP, RML, NIAID, NIH, 903 South 4th Street, Hamilton,
            MT 59840, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      71..659
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     repeat_region   790..973
                     /label=Borrelia burgdorferi IR2
                     /note="Borrelia burgdorferi IR2"
     CDS             complement(1178..1738)
                     /codon_start=1
                     /product="unknown"
                     /label=unknown
                     /note="Orf3; from Borrelia burgdorferi N40 plasmid cp9"
                     /protein_id="AAO62542.1"
                     /translation="MEKKRVVKVLTKKIDTYVEQNLMINESKISYYKTLKEKLNDNFKK
                     EIFHRVENIKILKEIKDNQYYKFDGYKTFLDFIKDFDVAKTQAYKYLRLATALQEGLIK
                     EDYLIENGIKNSYNFIKDKESPALKKSRQNPIKPLRFQLKTQESYDFYKKKSKFTSFMM
                     HEIFENQKDFLKKLLKKYEELKI"
     CDS             complement(1790..2341)
                     /codon_start=1
                     /product="unknown"
                     /label=unknown
                     /note="Orf2; from Borrelia burgdorferi N40 plasmid cp9"
                     /protein_id="AAO62541.1"
                     /translation="MKIGLKAARINEQKIKFPKESIFLLKEKKGDKNIYHTKILKALHR
                     VVSNKQKRCIIFFEKLLDKSEIQKLYLYPMKDGDKFLGIYYGYRKPINNVFVKYEINGV
                     ENVYTFSKIYYIEFRFKTGSVCCYLKNMRRLLRKEKIDTPYNKAIVDKFIDLEKHVYEF
                     YNKKYSSEGLILKWILKNLK"
     CDS             complement(2351..3478)
                     /codon_start=1
                     /product="unknown"
                     /label=unknown
                     /note="Orf1; from Borrelia burgdorferi N40 plasmid cp9"
                     /protein_id="AAO62540.1"
                     /translation="MENLSNINKTSKTTTCYNKLQYKLVVLISTLAYINKTYKKYTQKN
                     ILYYFNENLKRNSQTPVKLKTLQNYLYKLEKEFKVTTNYHKHLGVNLGTEIYYQLNFSK
                     KECYQKIYKYFQEKKDLRFQNRVSRGLKDRFTKNGSVDLKECLNNKNNIKEERKINEIE
                     KYQVRNYFNKCNFLCKKILSIFLTILFNLNIDKDNIIKILKIIKIIEIKLLKNKNIHFT
                     KSCMKEKQEKLKEILCNTQKEFEKNEYNSKQLETSFQKIYENYKFKPHFIIESHKYSDL
                     NNIKRKLEKSIERKKENSQQNYQDLKTNIFNILIEQLKKEVNIELLKPIIKEYLNNQKK
                     IEYNKVFCTYYCELLELMKNQKSLLSLKELNRKAI"
     repeat_region   3709..3892
                     /label=Borrelia burgdorferi IR1
                     /note="Borrelia burgdorferi IR1"
     protein_bind    4142..4163
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4178..4208
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4216..4232
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4240..4256
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4266..4322
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(4326..4342)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      4498..4907
                     /label=FlgB promoter
                     /note="FlgB promoter"
                     /regulatory_class="promoter"
     CDS             4908..5438
                     /label=GmR
                     /note="gentamycin acetyltransferase"