Basic Vector Information
- Vector Name:
- CIpNAT plasmid CIpNat
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7822 bp
- Type:
- Integrating vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Shahana S, Childers DS, Ballou ER, Bohovych I, Odds FC, Gow NA, Brown AJ.
- Promoter:
- TEF
CIpNAT plasmid CIpNat vector Map
CIpNAT plasmid CIpNat vector Sequence
LOCUS 40924_485 7822 bp DNA circular SYN 17-DEC-2018
DEFINITION Integrating vector CIpNAT plasmid CIpNat, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7822)
AUTHORS Shahana S, Childers DS, Ballou ER, Bohovych I, Odds FC, Gow NA,
Brown AJ.
TITLE New Clox Systems for Rapid and Efficient Gene Disruption in Candida
albicans
JOURNAL PLoS ONE 9 (6), E100390 (2014)
PUBMED 24940603
REFERENCE 2 (bases 1 to 7822)
AUTHORS Shahana S, Childers DS, Ballou ER, Bohovych I, Odds FC, Gow NA,
Brown AJ.
TITLE Direct Submission
JOURNAL Submitted (20-JAN-2014) School of Medical Sciences, University of
Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25
2ZD, UK
REFERENCE 3 (bases 1 to 7822)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7822)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
date: "2014"; volume: "9"; issue: "6"; pages: "E100390"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(20-JAN-2014) School of Medical Sciences, University of Aberdeen,
Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..7822
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 7..462
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 603..619
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 629..647
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_recomb 1040..1513
/label=RPS1 targeting sequence
/note="RPS1 targeting sequence"
primer_bind complement(1511..1527)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1544..1560
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1562..1595
/label=FRT (minimal)
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
promoter 1636..1979
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
CDS 1999..2562
/label=NrsR
/note="nourseothricin acetyltransferase"
terminator 2580..2777
/label=TEF terminator
/note="Ashbya gossypii TEF terminator"
regulatory complement(2810..3206)
/note="Candida albicans ACT1 terminator (Staib et al.,
1999)"
/regulatory_class="terminator"
CDS complement(3207..4475)
/label=FLP
/note="site-specific recombinase"
regulatory complement(4500..5542)
/gene="SAP2"
/note="Candida albicans SAP2 promoter (Staib et al., 1999)"
/regulatory_class="promoter"
gene complement(4500..5542)
/gene="SAP2"
/label=SAP2
primer_bind 5554..5570
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
misc_feature complement(5572..5605)
/note="FLP recombination target sequence (FRT) (Staib et
al., 1999)"
protein_bind 5572..5605
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
promoter complement(5638..5656)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5677..5693)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5701..5717)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5724..5754)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5769..5790)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6078..6666)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6840..7697)
/label=AmpR
/note="beta-lactamase"
promoter complement(7698..7802)
/label=AmpR promoter
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