AbVec-hIgG1 vector (V010129)

MSDS

Basic Vector Information

Vector Name:: AbVec-hIgG1

Antibiotic Resistance:: Ampicillin

Length:: 5746 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Di Niro R, Mesin L, Raki M, Zheng NY, Lund-Johansen F, Lundin KE, Charpilienne A, Poncet D, Wilson PC, Sollid LM.

Promoter:: SV40

AbVec-hIgG1 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

AbVec-hIgG1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_190        5746 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector AbVec-hIgG1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Di Niro R, Mesin L, Raki M, Zheng NY, Lund-Johansen F, Lundin KE, 
            Charpilienne A, Poncet D, Wilson PC, Sollid LM.
  TITLE     Rapid Generation of Rotavirus-Specific Human Monoclonal Antibodies 
            from Small-Intestinal Mucosa
  JOURNAL   J. Immunol. 185 (9), 5377-5383 (2010)
  PUBMED    20935207
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Wilson PC.
  TITLE     Antibody variable gene expression vector for human IgG1 heavy chain
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5746)
  AUTHORS   Wilson PC.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2008) Medicine/Rheumatology, The University of 
            Chicago, BSLC/JFK Bldg. 924 East 57th St., Rm 414, Chicago, IL 
            60637, USA
REFERENCE   4  (bases 1 to 5746)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5746)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Immunol."; date: "2010"; volume: "185"; issue: "9"; pages: 
            "5377-5383"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (18-NOV-2008) Medicine/Rheumatology, The University of Chicago, 
            BSLC/JFK Bldg. 924 East 57th St., Rm 414, Chicago, IL 60637, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             929..985
                     /label=IgH signal sequence
                     /note="signal sequence from the mouse immunoglobulin heavy
                     chain variable region"
     CDS             995..1981
                     /label=hlgG1-CH
                     /note="Human IgG1 heavy chain constant region"
     regulatory      1991..2000
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2004..2138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2211..2568
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(2588..2604)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2817..3272
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3604..3708
                     /label=AmpR promoter
     CDS             3709..4566
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4740..5328
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5616..5637
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5652..5682
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5690..5706
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5714..5730
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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