Basic Vector Information
- Vector Name:
- pET-22b-Tev-REV
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6162 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- E. coli
- Copy Number:
- Low
- Promoter:
- T7
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pET-22b-Tev-REV vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pET-22b-Tev-REV vector Sequence
LOCUS V013848 6162 bp DNA circular SYN 01-JAN-1980
DEFINITION Exported.
ACCESSION V013848
VERSION V013848
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 6162)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6162
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 12..467
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 494..598
/label="AmpR promoter"
CDS 599..1456
/label="AmpR"
/note="beta-lactamase"
rep_origin 1630..2218
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(2404..2546)
/label="bom"
/note="basis of mobility region from pBR322"
CDS complement(2651..2839)
/label="rop"
/note="Rop protein, which maintains plasmids at low copy
number"
protein_bind complement(3614..3635)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(3651..4730)
/label="lacI"
/note="lac repressor"
promoter complement(4731..4808)
/label="lacI promoter"
promoter 5117..5135
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 5136..5160
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 5175..5197
/label="RBS"
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
sig_peptide 5205..5270
/label="pelB signal sequence"
/note="leader peptide for secretion"
CDS 5301..5318
/label="6xHis"
/note="6xHis affinity tag"
CDS 5595..5615
/label="TEV site"
/note="tobacco etch virus (TEV) protease recognition and
cleavage site"
CDS 5631..5975
/gene="rev"
/label="Protein Rev"
/note="Protein Rev from Human immunodeficiency virus type 1
group M subtype B (isolate HXB2). Accession#: P04618"
CDS 6006..6023
/label="6xHis"
/note="6xHis affinity tag"
terminator 6090..6137
/label="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
This page is informational only.