Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V013698 | pAdEasy-fiber35 | In stock, 1 week for quality controls |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pAdEasy-fiber35
- Antibiotic Resistance:
- Ampicillin
- Length:
- 32702 bp
- Type:
- Packaging assistance
- Replication origin:
- ori
- Host:
- Mammalian cells, Adenovirus
- Copy Number:
- Low
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 30℃
pAdEasy-fiber35 vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pAdEasy-fiber35 vector Sequence
LOCUS V013698 32702 bp DNA circular UNA 01-JAN-1980
DEFINITION Exported.
ACCESSION V013698
VERSION V013698
KEYWORDS .
SOURCE natural DNA sequence
ORGANISM unspecified
.
REFERENCE 1 (bases 1 to 32702)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..32702
/mol_type="genomic DNA"
/organism="unspecified"
CDS 1291..1479
/label="rop"
/note="Rop protein, which maintains plasmids at low copy
number"
misc_feature 1584..1724
/label="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(1910..2498)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2672..3529)
/label="AmpR"
/note="beta-lactamase"
promoter complement(3530..3634)
/label="AmpR promoter"
CDS 3790..4209
/gene="IX"
/label="Hexon-interlacing protein"
/note="Hexon-interlacing protein from Human adenovirus C
serotype 5. Accession#: P03281"
CDS complement(4275..5609)
/gene="IVa2"
/label="Packaging protein 1"
/note="Packaging protein 1 from Human adenovirus C serotype
5. Accession#: P03271"
CDS complement(5381..8965)
/gene="POL"
/label="DNA polymerase"
/note="DNA polymerase from Human adenovirus C serotype 2.
Accession#: P03261"
CDS complement(8767..10770)
/gene="PTP"
/label="Preterminal protein"
/note="Preterminal protein from Human adenovirus C serotype
5. Accession#: P04499"
CDS 11229..12473
/gene="L1"
/label="Packaging protein 3"
/note="Packaging protein 3 from Human adenovirus C serotype
5. Accession#: P04496"
CDS 12497..14251
/note="Pre-hexon-linking protein IIIa from Human adenovirus
C serotype 5. Accession#: P12537"
/label="Pre-hexon-linking protein IIIa"
CDS 14335..16047
/gene="L2"
/label="Penton protein"
/note="Penton protein from Human adenovirus C serotype 5.
Accession#: P12538"
CDS 16057..16650
/gene="L2"
/label="Pre-histone-like nucleoprotein"
/note="Pre-histone-like nucleoprotein from Human adenovirus
C serotype 2. Accession#: P68950"
CDS 16723..17826
/gene="L2"
/label="Core-capsid bridging protein"
/note="Core-capsid bridging protein from Human adenovirus C
serotype 5. Accession#: P24938"
CDS 17857..18096
/note="Pre-core protein X from Human adenovirus C serotype
5. Accession#: Q2KS10"
/label="Pre-core protein X"
CDS 18182..18931
/gene="L3"
/label="Pre-protein VI"
/note="Pre-protein VI from Human adenovirus C serotype 5.
Accession#: P24937"
CDS 19020..21875
/gene="L3"
/label="Hexon protein"
/note="Hexon protein from Human adenovirus C serotype 5.
Accession#: P04133"
CDS 21911..22522
/gene="L3"
/label="Protease"
/note="Protease from Human adenovirus C serotype 5.
Accession#: P03253"
CDS complement(22624..24210)
/gene="DBP"
/label="DNA-binding protein"
/note="DNA-binding protein from Human adenovirus C serotype
5. Accession#: P03265"
CDS 24239..26659
/gene="L4"
/label="Shutoff protein"
/note="Shutoff protein from Human adenovirus C serotype 5.
Accession#: P24933"
CDS 27346..28026
/gene="L4"
/label="Pre-hexon-linking protein VIII"
/note="Pre-hexon-linking protein VIII from Human adenovirus
C serotype 5. Accession#: P24936"
CDS complement(29961..30842)
/note="Early 4 ORF6 protein from Human adenovirus C
serotype 2. Accession#: P03239"
/label="Early 4 ORF6 protein"
CDS complement(31120..31467)
/note="Early 4 ORF3 protein from Human adenovirus C
serotype 2. Accession#: P03241"
/label="Early 4 ORF3 protein"
CDS complement(31907..32290)
/note="Early 4 ORF1 protein from Human adenovirus C
serotype 2. Accession#: P03242"
/label="Early 4 ORF1 protein"
repeat_region 32600..32702
/label="ITR"
/note="inverted terminal repeat of human adenovirus
serotype 5"