Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V013465 | pBI121-35S-EGFP-PM | In stock, 1 week for quality controls |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pBI121-35S-EGFP-PM
- Antibiotic Resistance:
- Kanamycin
- Length:
- 15320 bp
- Type:
- Subcellular localization
- Replication origin:
- oriV
- Host:
- Plants
- Selection Marker:
- Neo/G418
- Promoter:
- CaMV35S(long)
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pBI121-35S-EGFP-PM vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pBI121-35S-EGFP-PM vector Sequence
LOCUS V013465 15320 bp DNA circular SYN 01-JAN-1980
DEFINITION Exported.
ACCESSION V013465
VERSION V013465
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 15320)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..15320
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(148..516)
/label="traJ"
/note="oriT-recognizing protein"
oriT complement(549..658)
/direction=LEFT
/label="oriT"
/note="incP origin of transfer"
CDS complement(1342..1989)
/label="TetR"
/note="tetracycline resistance regulatory protein"
misc_feature complement(2454..2478)
/label="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
promoter 2634..2817
/label="NOS promoter"
/note="nopaline synthase promoter"
CDS 2841..3629
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase from Tn5"
terminator 4022..4274
/label="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
rep_origin complement(4697..5321)
/direction=LEFT
/label="oriV"
/note="incP origin of replication"
promoter 5461..5806
/label="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 5837..6553
/label="EGFP"
/note="enhanced GFP"
CDS 6560..8269
/gene="NPF8.2"
/label="Protein NRT1/ PTR FAMILY 8.2"
/note="Protein NRT1/ PTR FAMILY 8.2 from Arabidopsis
thaliana. Accession#: Q9LFB8"
misc_feature complement(9184..9208)
/label="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
CDS complement(10807..11952)
/label="trfA"
/note="trans-acting replication protein that binds to and
activates oriV"
CDS complement(12254..13045)
/label="KanR"
/note="aminoglycoside phosphotransferase"
mobile_element 13237..14004
/label="IS1"
/note="prokaryotic transposable element"
rep_origin complement(14705..15315)
/direction=LEFT
/label="oriV"
/note="origin of replication for the bacterial F plasmid"