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pEGFP-FASTKD2 vector (V013272)

Basic Vector Information

Vector Name:
pEGFP-FASTKD2
Antibiotic Resistance:
Kanamycin
Length:
6833 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Selection Marker:
Neo/G418
Promoter:
CMV
Growth Strain(s):
DH5a
Growth Temperature:
37℃

pEGFP-FASTKD2 vector Vector Map

pEGFP-FASTKD26833 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600CMV enhancerCMV promoterFAST kinase domain-containing protein 2, mitochondrialEGFPSV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRHSV TK poly(A) signalori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pEGFP-FASTKD2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V013272                 6833 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013272
VERSION     V013272
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             625..2754
                     /gene="FASTKD2"
                     /label="FAST kinase domain-containing protein 2,
                     mitochondrial"
                     /note="FAST kinase domain-containing protein 2,
                     mitochondrial from Homo sapiens. Accession#: Q9NYY8"
     CDS             2779..3495
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    3621..3742
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3749..4204)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4231..4335
                     /label="AmpR promoter"
     promoter        4337..4694
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4729..5520
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5755..5802
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6131..6719
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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