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pENTR5-UbCp vector (V012987)

Basic Vector Information

Vector Name:
pENTR5-UbCp
Antibiotic Resistance:
Kanamycin
Length:
3861 bp
Type:
Protein expression
Replication origin:
ori
Host:
Mammalian cells
Promoter:
UbC
Growth Strain(s):
DB3.1
Growth Temperature:
37℃

pENTR5-UbCp vector Vector Map

pENTR5-UbCp3861 bp60012001800240030003600rrnB T1 terminatorM13 fwdattL4UbC promoterattR1T7 promoterM13 revKanRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pENTR5-UbCp vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_9490        3861 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(397..482)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     546..562
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    602..697
                     /label=attL4
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        698..1906
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     protein_bind    1906..2030
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2103..2121)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2126..2142)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2255..3061
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3211..3799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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