Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V012833 | pAAV-IRES-ZsGreen1 | In stock, instant shipping |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
ZsGreen1, a human codon-optimized variant of the green fluorescent protein from Zoanthus sp., can be used to monitor transfection and transduction efficiency.
- Vector Name:
- pAAV-IRES-ZsGreen1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5904 bp
- Type:
- Viral Expression & Packaging Vectors
- Replication origin:
- ori
- Selection Marker:
- ZsGreen1
- Promoter:
- CMV
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
pAAV-IRES-ZsGreen1 vector Vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pAAV-IRES-ZsGreen1 vector Sequence
LOCUS 40924_3165 5904 bp DNA circular SYN 19-DEC-2023
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5904)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..5904
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
enhancer 221..524
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 525..727
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 876..1254
/label=chimeric intron
/note="fusion between human cytomegalovirus intron A and
human beta-globin intron 2"
misc_feature 1345..1924
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 1919..2611
/label=ZsGreen1
/note="Zoanthus green fluorescent protein"
polyA_signal 2651..3127
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
repeat_region 3167..3307
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3382..3837
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4119..4223
/label=AmpR promoter
CDS 4224..5081
/label=AmpR
/note="beta-lactamase"
rep_origin 5255..5843
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"