pCMV-FLAG-N vector (V012826)

Price Information

Cat No. Plasmid Name Availability Add to cart
V012826 pCMV-FLAG-N In stock, 1 week for quality controls

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Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.

Basic Vector Information

Vector Name:
pCMV-FLAG-N
Antibiotic Resistance:
Ampicillin
Length:
3779 bp
Type:
Mammalian Expression Vectors
Replication origin:
ori
Copy Number:
High copy number
Promoter:
CMV
Growth Strain(s):
DH5alpha
Growth Temperature:
37℃

pCMV-FLAG-N vector Vector Map

pCMV-FLAG-N3779 bp60012001800240030003600CMV enhancerCMV promoterSV40 intronFLAGSV40 poly(A) signalM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterM13 fwd

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pCMV-FLAG-N vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pCMV-FLAG-N.        3779 bp DNA     circular SYN 04-NOV-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-FLAG-N
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          672..768
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             832..855
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     polyA_signal    complement(918..1052)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1163..1179)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1187..1203)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1211..1241)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1256..1277)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1565..2153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2327..3184)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3185..3289)
                     /label=AmpR promoter
     primer_bind     3763..3779
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"