pENTER vector (V012825)

MSDS

Basic Vector Information

Adenoviral pENTER shuttle vector with kanamycin selection and CMV promoter and C-terminal FLAG and His tags.

Vector Name:: pENTER

Antibiotic Resistance:: Kanamycin

Length:: 7509 bp

Type:: Mammalian Expression Vectors

Replication origin:: ori

Promoter:: CMV

Cloning Method:: Enzyme Cut

Fusion Tag:: FLAG

pENTER vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pENTER vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_17439        7509 bp DNA     circular SYN 04-NOV-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   21..123
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     211..361
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        419..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        954..972
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1073..1096
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1106..1123
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    1294..1415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             1509..1928
                     /gene="IX"
                     /label=IX
                     /note="Hexon-interlacing protein from Human adenovirus C 
                     serotype 5. Accession#: P03281"
     promoter        2342..2671
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2729..3325
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    3329..3553
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3771..4154)
                     /note="Early 4 ORF1 protein from Human adenovirus C
                     serotype 2. Accession#: P03242"
     repeat_region   4464..4566
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      complement(4838..5426)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             6258..7049
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(7100..7480)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"

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