pENTER vector (V012825)

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Basic Vector Information

Adenoviral pENTER shuttle vector with kanamycin selection and CMV promoter and C-terminal FLAG and His tags.

Vector Name:: pENTER

Antibiotic Resistance:: Kanamycin

Length:: 7509 bp

Type:: Mammalian Expression Vectors

Replication origin:: ori

Promoter:: CMV

Cloning Method:: Enzyme Cut

Fusion Tag:: FLAG

pENTER vector Map

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pENTER vector Sequence

LOCUS       V012825                 7509 bp    DNA     circular SYN 04-NOV-2023
DEFINITION  Exported.
ACCESSION   V012825
VERSION     V012825
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   21..123
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     211..361
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        419..722
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        954..972
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1073..1096
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1106..1123
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    1294..1415
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             1509..1928
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     promoter        2342..2671
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2729..3325
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     polyA_signal    3329..3553
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3771..4154)
                     /note="Early 4 ORF1 protein from Human adenovirus C
                     serotype 2. Accession#: P03242"
                     /label="Early 4 ORF1 protein"
     repeat_region   4464..4566
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      complement(4838..5426)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             6258..7049
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(7100..7480)
                     /direction=LEFT
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"

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