pAAV2/8 vector (V012818)

Price Information

Cat No. Plasmid Name Availability Add to cart
V012818 pAAV2/8 In stock (lyophilized plasmid)

Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

AAV packaging plasmid, expressing Rep2 and Cap8. This is a vector developed by Dr. James M. Wilson.

      • Vector Name:
      • pAAV2/8
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 7336 bp
      • Type:
      • Viral Expression & Packaging Vectors
      • Replication origin:
      • ori
      • Promoter:
      • Truncated P5
      • Growth Strain(s):
      • DH5alpha
      • Growth Temperature:
      • 37℃

pAAV2/8 vector Vector Map

pAAV2/87336 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300660069007200Rep78WELQut siteT3 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterf1 oriM13 fwdT7 promoterKS primer

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

  • Shastry S, Chu W, Barbieri E, et al. Rational design and experimental evaluation of peptide ligands for the purification of adeno-associated viruses via affinity chromatography. Biotechnol J. 2024;19(1):e2300230. doi:10.1002/biot.202300230

pAAV2/8 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_3244        7336 bp DNA     circular SYN 21-AUG-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1863
                     /gene="Rep78"
                     /label=Rep78
                     /note="Protein Rep78 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89268"
     CDS             3941..3952
                     /label=WELQut site
                     /note="WELQut protease recognition and cleavage site"
     promoter        complement(4404..4422)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4443..4459)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4467..4483)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4491..4521)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4536..4557)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4845..5433)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5607..6464)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6465..6569)
                     /label=AmpR promoter
     rep_origin      complement(6595..7050)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7192..7208
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7218..7236
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7266..7282
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"