pGEM-HE vector (V012814#)

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V012814	pGEM-HE	In stock (lyophilized plasmid)	Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

Xenopus oocyte expression vector. It contains a 3'-UTR of the major beta-globin gene of Xenopus laevis. The pGEM-HE is a derivative of pGEM3Z vector.

- - Vector Name:
  - pGEM-HE
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 3047 bp
- - Type:
  - Protein Expression plasmid
- - Selection Marker:
  - Ampicillin, 100 μg/mL
- - Copy Number:
  - High copy number
- - Promoter:
  - T7
- - Growth Strain(s):
  - DH5alpha
- - Growth Temperature:
  - 37℃

pGEM-HE vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

Loewen SK, Yao SY, Slugoski MD, Mohabir NN, Turner RJ, Mackey JR, Weiner JH, Gallagher MP, Henderson PJ, Baldwin SA, Cass CE, Young JD. Transport of physiological nucleosides and anti-viral and anti-neoplastic nucleoside drugs by recombinant Escherichia coli nucleoside-H(+) cotransporter (NupC) produced in Xenopus laevis oocytes. Mol Membr Biol. 2004 Jan-Feb;21(1):1-10.

pGEM-HE vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                3047 bp ds-DNA     circular SYN 03-AUG-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pGEM-HE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3047)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     46..62
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        69..87
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           119..161
                     /note="Xenopus globin 5'-UTR"
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     3'UTR           220..345
                     /note="Xenopus globin 3'-UTR"
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     promoter        complement(457..475)
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(493..509)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    517..533
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(541..571)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    586..607
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(895..1483)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1654..2514)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2515..2619)
                     /gene="bla"
                     /note="AmpR promoter"