Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V012812 | pAAV-Ef1a-fDIO EYFP | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
EF1a-driven, Flp-dependent expression of EYFP.
pAAV-Ef1a-fDIO EYFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pAAV-Ef1a-fDIO EYFP vector Sequence
LOCUS Exported 6313 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Flp-Dependent EYFP.
ACCESSION .
VERSION .
KEYWORDS pAAV-Ef1a-fDIO EYFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6313)
AUTHORS Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone
J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H,
Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ,
Deisseroth K
TITLE Targeting cells with single vectors using multiple-feature Boolean
logic.
JOURNAL Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub
2014 Jun 8.
PUBMED 24908100
REFERENCE 2 (bases 1 to 6313)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6313
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..1179
/note="EF-1-alpha promoter"
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 232..1170
/note="EF-1-alpha intron A"
/note="intron upstream of the start codon of human
EF-1-alpha"
protein_bind 1210..1243
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FRT (minimal)"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
CDS complement(1336..2055)
/codon_start=1
/product="enhanced YFP"
/note="EYFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
regulatory 2052..2061
/regulatory_class="other"
/note="Kozak sequence"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
protein_bind complement(2068..2101)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FRT (minimal)"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
misc_feature 2210..2798
/note="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2681..2692)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/note="Factor Xa site"
/translation="IEGR"
polyA_signal 2830..3306
/note="hGH poly(A) signal"
/note="human growth hormone polyadenylation signal"
repeat_region 3346..3486
/note="AAV2 ITR"
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3346..3475
/note="AAV2 ITR"
rep_origin 3561..4016
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4298..4402
/gene="bla"
/note="AmpR promoter"
CDS 4403..5263
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5434..6022
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
repeat_region 6084..6213
/note="AAV2 ITR (alternate)"
/note="Functional equivalent of wild-type AAV2 ITR"