pSV-SPORT vector (V012808)

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V012808	pSV-SPORT	In stock (lyophilized plasmid)	Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

- - Vector Name:
  - pSV-SPORT
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 3169 bp
- - Type:
  - Mammalian Expression Vectors
- - Copy Number:
  - High copy number
- - Promoter:
  - SV40
- - Cloning Method:
  - Enzyme Cut
- - 5' Primer:
  - SP6
- - 3' Primer:
  - T7
- - Expression Method:
  - Transient

pSV-SPORT vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pSV-SPORT vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                3169 bp ds-DNA     circular SYN 03-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSV-SPORT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Tontonoz P, Kim JB, Graves RA, Spiegelman BM
  TITLE     ADD1: a novel helix-loop-helix transcription factor associated with 
            adipocyte determination and differentiation.
  JOURNAL   Mol Cell Biol. 1993 Aug . 13(8):4753-9.
  PUBMED    8336713
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        10..338
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      189..324
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        344..362
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(477..495)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    882..1016
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1253..1841)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2012..2872)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2873..2977)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        3090..3120
                     /note="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    3128..3144
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."