Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V012808 | pSV-SPORT | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
| Basic Vector Information | |||
|---|---|---|---|
| Vector Name | pSV-SPORT | Antibiotic Resistance | Ampicillin |
| Length | 3169 bp | Type | Mammalian Expression Vectors |
| Copy Number | High copy number | Promoter | SV40 |
| Cloning Method | Enzyme Cut | 5' Primer | SP6 |
| 3' Primer | T7 | Expression Method | Transient |
pSV-SPORT vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pSV-SPORT vector Sequence
LOCUS Exported 3169 bp ds-DNA circular SYN 03-FEB-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pSV-SPORT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3169)
AUTHORS Tontonoz P, Kim JB, Graves RA, Spiegelman BM
TITLE ADD1: a novel helix-loop-helix transcription factor associated with
adipocyte determination and differentiation.
JOURNAL Mol Cell Biol. 1993 Aug . 13(8):4753-9.
PUBMED 8336713
REFERENCE 2 (bases 1 to 3169)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..3169
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 10..338
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 189..324
/note="SV40 ori"
/note="SV40 origin of replication"
promoter 344..362
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
promoter complement(477..495)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 882..1016
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1253..1841)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2012..2872)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2873..2977)
/gene="bla"
/note="AmpR promoter"
promoter 3090..3120
/note="lac UV5 promoter"
/note="E. coli lac promoter with an ""up"" mutation"
protein_bind 3128..3144
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."