Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V012808 | pSV-SPORT | In stock, instant shipping |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pSV-SPORT
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3169 bp
- Type:
- Mammalian Expression Vectors
- Replication origin:
- ori
- Copy Number:
- High copy number
- Promoter:
- lac UV5
- Cloning Method:
- Enzyme Cut
- 5' Primer:
- SP6
- 3' Primer:
- T7
- Growth Strain(s):
- stbl3
- Growth Temperature:
- 37℃
- Expression Method:
- Transient
pSV-SPORT vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pSV-SPORT vector Sequence
LOCUS pSV-SPORT. 3169 bp DNA circular SYN 03-FEB-2023
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS pSV-SPORT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3169)
AUTHORS Tontonoz P, Kim JB, Graves RA, Spiegelman BM
TITLE ADD1: a novel helix-loop-helix transcription factor associated with
adipocyte determination and differentiation.
JOURNAL Mol Cell Biol. 1993 Aug . 13(8):4753-9.
PUBMED 8336713
REFERENCE 2 (bases 1 to 3169)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell
Biol. 1993 Aug . 13(8):4753-9."
FEATURES Location/Qualifiers
source 1..3169
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 10..338
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
promoter 344..362
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
promoter complement(477..495)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 882..1016
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1253..1841)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2015..2872)
/label=AmpR
/note="beta-lactamase"
promoter complement(2873..2977)
/label=AmpR promoter
promoter 3090..3120
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
protein_bind 3128..3144
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter 3169
/label=SV40
/note="Simian virus 40 enhancer/early promoter"