Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V012806 | pKEW303-AcrVA5 | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
Expresses AcrVA5 in bacteria, under TetR control.
pKEW303-AcrVA5 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pKEW303-AcrVA5 vector Sequence
LOCUS Exported 3198 bp ds-DNA circular SYN 31-AUG-2022
DEFINITION Expresses AcrVA5 in bacteria, under TetR control..
ACCESSION .
VERSION .
KEYWORDS pKEW303-AcrVA5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3198)
AUTHORS Watters KE, Fellmann C, Bai HB, Ren SM, Doudna JA
TITLE Systematic discovery of natural CRISPR-Cas12a inhibitors.
JOURNAL Science. 2018 Oct 12;362(6411):236-239. doi:
10.1126/science.aau5138. Epub 2018 Sep 6.
PUBMED 30190307
REFERENCE 2 (bases 1 to 3198)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..3198
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..56
/gene="tetR"
/note="tetR/tetA promoters"
/note="overlapping promoters for bacterial tetR and tetA"
protein_bind 37..55
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="tet operator"
/note="bacterial operator O2 for the tetR and tetA genes"
CDS 83..361
/codon_start=1
/note="anti-CRISPR N-acetyltransferase AcrVA5"
/translation="MKIELSGGYICYSIEEDEVTIDMVEVTTKRQGIGSQLIDMVKDVA
REVGLPIGLYAYPQDDSISQEDLIEFYFSNDFEYDPDDVDGRLMRWS"
terminator 594..621
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
rep_origin complement(747..1335)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1506..2366)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2367..2471)
/gene="bla"
/note="AmpR promoter"
CDS complement(2557..3180)
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/note="TetR"
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"