pBBR1MCS2-Tac-mCherry vector (V012805#)

Price Information

Cat No. Plasmid Name Availability Add to cart
V012805 pBBR1MCS2-Tac-mCherry In stock (lyophilized plasmid)

Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

red fluorescent protein expression plasmid; wide host plasmid.

      • Vector Name:
      • pBBR1MCS2-Tac-mCherry
      • Antibiotic Resistance:
      • Kanamycin
      • Length:
      • 6195 bp
      • Type:
      • Expression plasmid
      • Promoter:
      • Tac
      • Cloning Method:
      • Enzyme Cut
      • 5' Primer:
      • 3' Primer:
      • Fusion Tag:
      • mCherry

pBBR1MCS2-Tac-mCherry vector Vector Map

pBBR1MCS2-Tac-mCherry6195 bp30060090012001500180021002400270030003300360039004200450048005100540057006000NeoR/KanRCAP binding sitelac promoterlac operatorM13 revT3 promoterKS primerlacI promotertac promotermCherryT7 terminatorSK primerT7 promoterM13 fwdpBBR1 ReppBBR1 oriV

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pBBR1MCS2-Tac-mCherry vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                6195 bp ds-DNA     circular SYN 15-JUL-2022
DEFINITION  synthetic circular DNA
KEYWORDS    pBBR1MCS2-Tac-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6195)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             458..1252
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="confers resistance to neomycin, kanamycin, and G418 
     protein_bind    1657..1678
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1693..1723
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1731..1747
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1755..1771
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
     promoter        1792..1810
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1840..1856
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
     promoter        complement(1910..1987)
                     /note="lacI promoter"
     misc_feature    2033..2061
                     /note="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             2108..2818
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mammalian codon-optimized"
     terminator      2852..2899
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
     primer_bind     complement(2941..2957)
                     /note="SK primer"
                     /note="common sequencing primer, one of multiple similar 
     promoter        complement(2990..3008)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3018..3034)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
     CDS             complement(3741..4403)
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /note="pBBR1 Rep"
     rep_origin      4404..5173
                     /note="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"