Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V012805 | pBBR1MCS2-Tac-mCherry | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
Red fluorescent protein expression plasmid; wide host plasmid.
pBBR1MCS2-Tac-mCherry vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Geng Z, Wang X, Wu F, Cao Z, Liu J. Biointerface mineralization generates ultraresistant gut microbes as oral biotherapeutics. Sci Adv. 2023 Mar 15;9(11):eade0997.
pBBR1MCS2-Tac-mCherry vector Sequence
LOCUS pBBR1MCS2-Tac-mC 6195 bp DNA circular SYN 15-JUL-2022
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS pBBR1MCS2-Tac-mCherry.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6195)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6195
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 458..1249
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
protein_bind 1657..1678
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 1693..1723
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1731..1747
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 1755..1771
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 1792..1810
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind 1840..1856
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(1910..1987)
/label=lacI promoter
promoter 2033..2061
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
CDS 2108..2815
/label=mCherry
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
terminator 2852..2899
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind complement(2941..2957)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(2990..3008)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3018..3034)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(3744..4403)
/label=pBBR1 Rep
/note="replication protein for the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica"
rep_origin complement(4404..5173)
/direction=LEFT
/label=pBBR1 oriV
/note="replication origin of the broad-host-range plasmid
pBBR1 from Bordetella bronchiseptica; requires the pBBR1
Rep protein for replication"