|Cat No.||Plasmid Name||Availability||Add to cart|
|V012803||pMG36e||In stock (lyophilized plasmid)||
Buy one, get one free!
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
pMG36e plasmid (pWV01 ori) is based on protease gene transcription and translation signals from Lactococcus lactis cremoris, is a typical artificial constitutive expression vector as a main expression system in Lactococcus.It contains a strong promoter and can express foreign proteins in a variety of bacteria. It has been used to study the action mechanism of bacteriocin, the transformation of lactic acid bacteria genetic engineering strains and the development of oral vaccines. It has been widely used in many fields and has become one of the important tools for lactic acid bacteria genetic engineering research.
pMG36e vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMG36e vector Sequence
LOCUS Exported 3610 bp ds-DNA circular SYN 17-JUN-2022 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS pMG36e SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3610) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..3610 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 227..925 /codon_start=1 /note="repA" /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE KKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK VLDAETGEIK" CDS complement(1463..2197) /codon_start=1 /note="Emr" /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT LELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPY NISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPREYF HPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID DLNNISFEQFLSLFNSYKLFNK" promoter 2474..2652 /note="P32" CDS 2653..2892 /codon_start=1 /note="ORF" /translation="MAIVSAEKFVIRARPGIDPLESTCRHASLQSLKTKVAAAVEAAKT VGKGDGTTGTSDKGGGQGTPALRYLELRFKVKWY"