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pGro7 vector (V012802)

Price Information

Cat No. Plasmid Name Availability Add to cart
V012802 pGro7 In stock (lyophilized plasmid)

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Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

Plasmids of chaperone. The function of the plasmid pGro7 is to express the GroEL and GroES proteins, which are chaperonins involved in protein folding in the cytoplasm of Escherichia coli. pGro7 is a high-copy-number plasmid, meaning that it exists in multiple copies per cell, and it is often used for overexpression of proteins in E. coli. The GroEL and GroES proteins form a complex that helps to fold newly synthesized proteins into their correct conformation, preventing protein aggregation and enhancing protein stability. pGro7 is a valuable tool in molecular biology for protein production and research.

Vector Name:
pGro7
Antibiotic Resistance:
Chloramphenicol
Length:
5467 bp
Type:
E.coli expression plasmid
Replication origin:
p15A ori
Source/Author:
Takara
Copy Number:
Medium copy number
Growth Strain(s):
Stbl3
Growth Temperature:
37℃

pGro7 vector Vector Map

pGro75467 bp60012001800240030003600420048005400S loopgroELp15A oricat promoterCmR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

  • Pei C, Peng X, Wu Y, Jiao R, Li T, Jiao S, Zhou L, Li J, Du Y, Qian EW. Characterization and application of active human α2,6-sialyltransferases ST6GalNAc V and ST6GalNAc VI recombined in Escherichia coli. Enzyme Microb Technol. 2024 Mar 12;177:110426. doi: 10.1016/j.enzmictec.2024.110426. Epub ahead of print. PMID: 38503081.

pGro7 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pGro7.        5467 bp DNA     circular SYN 10-JUN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pGro7.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5467)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1862..1915
                     /label=S loop
                     /note="GroES chaperone mobile loop that interacts with
                     GroEL"
     CDS             2154..3797
                     /gene="groEL"
                     /label=groEL
                     /note="Chaperonin GroEL from Escherichia coli O8 (strain
                     IAI1). Accession#: B7M8Q4"
     rep_origin      4076..4620
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        5146..5248
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             join(5249..5467,1..438)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"