Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V012799 | pBBR1MCS5-Tac-EGFP | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
pBBR1MCS5-Tac-EGFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pBBR1MCS5-Tac-EGFP vector Sequence
LOCUS 40924_6073 5539 bp DNA circular SYN 18-APR-2022 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5539) AUTHORS NOVOPRO TITLE Direct Submission FEATURES Location/Qualifiers source 1..5539 /mol_type="other DNA" /organism="synthetic DNA construct" rep_origin 1023..1792 /label=pBBR1 oriV /note="replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication" CDS 1793..2452 /label=pBBR1 Rep /note="replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica" primer_bind 3162..3178 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 3188..3206 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 3239..3255 /label=SK primer /note="common sequencing primer, one of multiple similar variants" promoter 3257..3285 /label=tac promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" CDS 3315..4031 /label=EGFP /note="enhanced GFP" primer_bind complement(4060..4076) /label=KS primer /note="common sequencing primer, one of multiple similar variants" promoter complement(4106..4124) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4145..4161) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(4169..4185) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4193..4223) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4238..4259) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 4529..4557 /label=Pc promoter /note="class 1 integron promoter" CDS 4746..5276 /label=GmR /note="gentamycin acetyltransferase"