pBBR1MCS5-Tac-EGFP vector (V012799)

Price Information

Cat No. Plasmid Name Availability Add to cart
V012799 pBBR1MCS5-Tac-EGFP In stock (lyophilized plasmid)

Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

      • Vector Name:
      • pBBR1MCS5-Tac-EGFP
      • Antibiotic Resistance:
      • Gentamicin
      • Length:
      • 5539 bp
      • Type:
      • Expression plasmid
      • Copy Number:
      • High copy number
      • Promoter:
      • Tac
      • Cloning Method:
      • Enzyme Cut
      • 3' Primer:
      • M13 rev
      • Fusion Tag:
      • EGFP

pBBR1MCS5-Tac-EGFP vector Vector Map

pBBR1MCS5-Tac-EGFP5539 bp60012001800240030003600420048005400pBBR1 oriVpBBR1 RepM13 fwdT7 promoterSK primertac promoterEGFPKS primerT3 promoterM13 revlac operatorlac promoterCAP binding sitePc promoterGmR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pBBR1MCS5-Tac-EGFP vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_6073        5539 bp DNA     circular SYN 18-APR-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   NOVOPRO
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2452
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     primer_bind     3162..3178
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3188..3206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3239..3255
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3257..3285
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             3315..4031
                     /label=EGFP
                     /note="enhanced GFP"
     primer_bind     complement(4060..4076)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4106..4124)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4145..4161)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4169..4185)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4193..4223)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4238..4259)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4529..4557
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4746..5276
                     /label=GmR
                     /note="gentamycin acetyltransferase"