Cart 2

ppSUMO vector (V012798)

Basic Vector Information

Also named pET28a-SUMO.

Vector Name:
ppSUMO
Antibiotic Resistance:
Kanamycin
Length:
5633 bp
Type:
Bacterial expression vector
Replication origin:
ori
Copy Number:
High copy number
Promoter:
T7
Cloning Method:
Enzyme Cut
5' Primer:
T7 promoter
3' Primer:
T7 terminator
Fusion Tag:
His & SUMO
Expression Method:
IPTG induced

ppSUMO vector Map

Copy Sequence View Map
ppSUMO5633 bp60012001800240030003600420048005400f1 oriKanRoribomropCAP binding sitelacIlacI promoterT7 promoterlac operatorRBS6xHisthrombin siteSUMO6xHisT7 terminator

ppSUMO vector Sequence

LOCUS       Untitled.        5633 bp DNA     circular SYN 05-MAR-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    Untitled
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   hB
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(563..1375)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1497..2085
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2271..2413)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2518..2706)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(3481..3502)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3518..4597)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(4598..4675)
                     /label=lacI promoter
     promoter        4984..5002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5042..5064
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5083..5100
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             5110..5127
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             5140..5433
                     /label=SUMO
                     /note="cleavable ubiquitin-like protein tag"
     CDS             5477..5494
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      5561..5608
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"

This page is informational only.