pacAd5 CMVK-NpA vector (V012788)

Basic Vector Information

pacAd5 K-NpA shuttle vector does not contain a promoter ahead of the multiple cloning sites. You must clone a promoter into the vector along with your gene of interest.

Vector Name:: pacAd5 CMVK-NpA

Antibiotic Resistance:: Ampicillin

Length:: 6215 bp

Type:: Adenoviral Expression Vector

Replication origin:: ori

Source/Author:: Cell BioLabs

Copy Number:: High copy number

Promoter:: CMV

Cloning Method:: Enzyme Cut

pacAd5 CMVK-NpA vector Map

Copy Sequence View Map

pacAd5 CMVK-NpA vector Sequence

LOCUS       V012788                 6215 bp    DNA     circular SYN 17-NOV-2021
DEFINITION  Exported.
ACCESSION   V012788
VERSION     V012788
KEYWORDS    Untitled
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6215)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   16..118
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     206..356
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        385..688
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        689..892
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     928..944
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(978..994)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     polyA_signal    complement(1014..1148)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             1716..2135
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(2201..3535)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     rep_origin      complement(4388..4976)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5150..6007)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6008..6112)
                     /label="AmpR promoter"

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