Basic Vector Information
- Vector Name:
- pacAd5 CMV-GFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6935 bp
- Type:
- Adenoviral Expression Vector
- Replication origin:
- ori
- Source/Author:
- Cell BioLabs
- Copy Number:
- High copy number
- Promoter:
- CMV
- Cloning Method:
- Enzyme Cut
- Fusion Tag:
- EGFP
pacAd5 CMV-GFP vector Map
pacAd5 CMV-GFP vector Sequence
LOCUS V012787 6935 bp DNA circular SYN 17-NOV-2021
DEFINITION Exported.
ACCESSION V012787
VERSION V012787
KEYWORDS pacAd5 CMV-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 6935)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 6935)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6935
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 16..118
/label="ITR"
/note="inverted terminal repeat of human adenovirus
serotype 5"
misc_signal 206..356
/label="Ad5 Psi"
/note="packaging signal for adenovirus serotype 5"
enhancer 385..688
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 689..892
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 926..978
/label="MCS"
/note="multiple cloning site"
CDS 992..1708
/label="EGFP"
/note="enhanced GFP"
polyA_signal complement(1732..1866)
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
CDS 2436..2855
/gene="IX"
/label="Hexon-interlacing protein"
/note="Hexon-interlacing protein from Human adenovirus C
serotype 5. Accession#: P03281"
CDS complement(2921..4255)
/gene="IVa2"
/label="Packaging protein 1"
/note="Packaging protein 1 from Human adenovirus C serotype
5. Accession#: P03271"
rep_origin complement(5108..5696)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5870..6727)
/label="AmpR"
/note="beta-lactamase"
promoter complement(6728..6832)
/label="AmpR promoter"
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