pacAd5 CMV-GFP vector (V012787)

MSDS

Basic Vector Information

- - Vector Name:
  - pacAd5 CMV-GFP
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 6935 bp
- - Type:
  - Adenoviral Expression Vector
- - Replication origin:
  - ori
- - Source/Author:
  - Cell BioLabs
- - Copy Number:
  - High copy number
- - Promoter:
  - CMV
- - Cloning Method:
  - Enzyme Cut
- - Fusion Tag:
  - EGFP

pacAd5 CMV-GFP vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pacAd5 CMV-GFP vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pacAd5_CMV-GFP.        6935 bp DNA     circular SYN 17-NOV-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pacAd5 CMV-GFP.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6935)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   16..118
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     206..356
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        385..688
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        689..892
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    926..978
                     /label=MCS
                     /note="multiple cloning site"
     CDS             992..1708
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    complement(1732..1866)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             2436..2855
                     /gene="IX"
                     /label=IX
                     /note="Hexon-interlacing protein from Human adenovirus C 
                     serotype 5. Accession#: P03281"
     CDS             complement(2921..4255)
                     /gene="IVa2"
                     /label=IVa2
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     rep_origin      complement(5108..5696)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5870..6727)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6728..6832)
                     /label=AmpR promoter

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