pacAd5 CMV-GFP vector (V012787)

MSDS

Basic Vector Information

Vector Name:: pacAd5 CMV-GFP

Antibiotic Resistance:: Ampicillin

Length:: 6935 bp

Type:: Adenoviral Expression Vector

Replication origin:: ori

Source/Author:: Cell BioLabs

Copy Number:: High copy number

Promoter:: CMV

Cloning Method:: Enzyme Cut

Fusion Tag:: EGFP

pacAd5 CMV-GFP vector Vector Map

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pacAd5 CMV-GFP vector Sequence

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LOCUS       V012787                 6935 bp    DNA     circular SYN 17-NOV-2021
DEFINITION  Exported.
ACCESSION   V012787
VERSION     V012787
KEYWORDS    pacAd5 CMV-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6935)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   16..118
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     206..356
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        385..688
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        689..892
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    926..978
                     /label="MCS"
                     /note="multiple cloning site"
     CDS             992..1708
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    complement(1732..1866)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             2436..2855
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(2921..4255)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     rep_origin      complement(5108..5696)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5870..6727)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6728..6832)
                     /label="AmpR promoter"

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