Basic Vector Information
pTRV2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTRV2 vector Sequence
LOCUS pTRV2. 9663 bp DNA circular SYN 18-SEP-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS pTRV2. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9663) TITLE Direct Submission REFERENCE 2 (bases 1 to 9663) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..9663 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 561..1172 /gene="CP" /label=CP /note="Capsid protein from Tobacco rattle virus (isolate PpK20). Accession#: Q88897" misc_feature 1709..2103 /label=TRV strain ppk20 RNA2 3'-sequence /note="TRV strain ppk20 RNA2 3'-sequence" CDS 2164..2181 /label=6xHis /note="6xHis affinity tag" terminator 2216..2468 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" misc_feature 2490..2514 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 3814..4440 /label=pVS1 StaA /note="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS 4877..5941 /label=pVS1 RepA /note="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" rep_origin 6010..6204 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" misc_feature 6548..6688 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(6874..7462) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7552..8343) /label=KanR /note="aminoglycoside phosphotransferase" misc_feature 8768..8792 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" promoter 8990..9663 /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region"
This page is informational only.