Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V012780 | pTRV2 | In stock, 1 week for quality controls |
Buy one, get one free! |
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.
Basic Vector Information
- Vector Name:
- pTRV2
- Antibiotic Resistance:
- Kanamycin
- Length:
- 9663 bp
- Type:
- RNAi plasmid
- Replication origin:
- ori
- Promoter:
- CaMV35S(enhanced)
- Cloning Method:
- Enzyme Cut
pTRV2 vector Map
Plasmid Protocol
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5. Store the plasmid at -20 ℃.
6. The concentration of plasmid re-measurement sometimes differs from the nominal value, which may be due to the position of the lyophilized plasmid in the tube, the efficiency of the re-dissolution, the measurement bias, and adsorption on the wall of the tube, therefore, it is recommended to transform and extract the plasmid before using it
General Plasmid Transform Protocol
1. Take one 100μl of the competent cells and thaw it on ice for 10min, add 2μl of plasmid, then ice bath for 30min, then heat-shock it at 42℃ for 60s, do not stir, and then ice bath for 2min.
2. Add 900μl of LB liquid medium without antibiotics, and incubate at 37℃ for 45min (30℃ for 1-1.5 hours) with 180rpm shaking.
3. Centrifuge at 6000rpm for 5min, leave only 100μl of supernatant to resuspend the bacterial precipitate and spread it onto the target plasmid-resistant LB plate.
4. Invert the plate and incubate at 37℃ for 14h, or at 30℃ for 20h.
5. Pick a single colony into LB liquid medium, add the corresponding antibiotics, incubate at 220rpm for 14h, and extract the plasmid according to the experimental needs and the instructions of the plasmid extraction kit.
pTRV2 vector Sequence
LOCUS V012780 9663 bp DNA circular SYN 18-SEP-2021
DEFINITION Exported.
ACCESSION V012780
VERSION V012780
KEYWORDS pTRV2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 9663)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 9663)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9663
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 561..1172
/gene="CP"
/label="Capsid protein"
/note="Capsid protein from Tobacco rattle virus (isolate
PpK20). Accession#: Q88897"
misc_feature 1709..2103
/label="TRV strain ppk20 RNA2 3'-sequence"
/note="TRV strain ppk20 RNA2 3'-sequence"
CDS 2164..2181
/label="6xHis"
/note="6xHis affinity tag"
terminator 2216..2468
/label="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 2490..2514
/label="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
CDS 3814..4440
/label="pVS1 StaA"
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 4877..5941
/label="pVS1 RepA"
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 6010..6204
/label="pVS1 oriV"
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 6548..6688
/label="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(6874..7462)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7552..8343)
/label="KanR"
/note="aminoglycoside phosphotransferase"
misc_feature 8768..8792
/label="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
promoter 8990..9663
/label="CaMV 35S promoter (enhanced)"
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"