pTRV2 vector (V012780)

Basic Vector Information

      • Vector Name:
      • pTRV2
      • Antibiotic Resistance:
      • Kanamycin
      • Length:
      • 9663 bp
      • Type:
      • RNAi plasmid
      • Promoter:
      • CaMV 35S promoter
      • Cloning Method:
      • Enzyme Cut

pTRV2 vector Vector Map

pTRV29663 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400880092009600CPTRV strain ppk20 RNA2 3'-sequence6xHisNOS terminatorRB T-DNA repeatpVS1 StaApVS1 RepApVS1 oriVbomoriKanRLB T-DNA repeatCaMV 35S promoter (enhanced)

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTRV2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pTRV2.        9663 bp DNA     circular SYN 18-SEP-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pTRV2.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9663)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9663)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             561..1172
                     /gene="CP"
                     /label=CP
                     /note="Capsid protein from Tobacco rattle virus (isolate
                     PpK20). Accession#: Q88897"
     misc_feature    1709..2103
                     /label=TRV strain ppk20 RNA2 3'-sequence
                     /note="TRV strain ppk20 RNA2 3'-sequence"
     CDS             2164..2181
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      2216..2468
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2490..2514
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3814..4440
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             4877..5941
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6010..6204
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6548..6688
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6874..7462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7552..8343)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    8768..8792
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        8990..9663
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"

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