Basic Vector Information
All pEF or pUB vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag.
- Vector Name:
- pUB6/V5-His B
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5467 bp
- Type:
- Mammalian Expression Vectors
- Replication origin:
- ori
- Source/Author:
- Invitrogen
- Selection Marker:
- Blasticidin
- Copy Number:
- High copy number
- Promoter:
- UbC
- Cloning Method:
- Enzyme Cut
- 5' Primer:
- T7 Fwd:5'd[TAATACGACTCACTATAGGG]3'
- Fusion Tag:
- His Tag (6x), V5 Epitope Tag
- Expression Method:
- Constiutive, Stable / Transient
pUB6/V5-His B vector Vector Map
pUB6/V5-His B vector Sequence
LOCUS 40924_44844 5467 bp DNA circular SYN 18-SEP-2021
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5467)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 5467)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5467
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 18..1227
/label=UbC promoter
/note="human ubiquitin C promoter"
CDS 1331..1372
/codon_start=1
/label=V5 tag
/note="epitope tag from simian virus 5"
/translation="GKPIPNPLLGLDST"
CDS 1382..1399
/codon_start=1
/label=6xHis
/note="6xHis affinity tag"
/translation="HHHHHH"
polyA_signal 1428..1652
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1698..2126
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2140..2470
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
promoter 2518..2565
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 2584..2979
/codon_start=1
/label=BSD
/note="blasticidin S deaminase"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
polyA_signal 3140..3273
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3310..3326)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(3334..3350)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3358..3388)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(3403..3424)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3712..4300)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4474..5331)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5332..5436)
/label=AmpR promoter
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